Organizations are contending with various challenges such as increasing global competition, changing customer preferences, dramatic advances in the field of information and communication technology and formation of various trading blocks around the world. Human resource has been considered as one of the most important factors that enable firms to compete effectively in such market place environments. The aim of this study was to examine the impact of human resource management practices on employees’ performance. The study was conducted through a case study of Serena hotel in Arusha. Data was collected through a structured questionnaire, from 64 respondents out of the 75 employees. The study established that the recruitment and selection activity was the best implemented practice matching 90.9% of the employees’ expectations. Training and development were perceived as the second-best practice with a 72.7% success followed by performance appraisal at 63.7%. Reward and compensation practices had a moderate impact on employees’ performance as it was rated at 31.9% satisfaction level in relation to employee’s expectations. The study concluded that effective human resource practices can influence employees’ performance if the interests and expectations of the employees are considered during policy formulation and implementation in an organization. The results confirm that effective HRM practices can guarantee a high level of employee satisfaction which in turn influences individual and group performance. In light of these findings, the study recommends that organizations prioritize comprehensive HRM practices designed to enhance employee engagement through tailored training programs, and cultivate a positive workplace culture that encourages open communication and recognizes employee contributions.