Background: Maternal syphilis remains under-recognized in Sudan despite its contribution to poor pregnancy outcomes. Local prevalence data are needed to inform targeted interventions. Objective: To determine the prevalence of syphilis and associated demographic and clinical characteristics among pregnant women in Al-Nuhod locality, Sudan. Methods: A descriptive cross-sectional study was conducted among 400 pregnant women from 2018–2021. Syphilis testing was performed using rapid plasma reagin (RPR). Demographic and clinical characteristics, including age, education, marital status, reproductive history, and ANC attendance, were recorded using structured questionnaires. Results: The overall prevalence of syphilis was 0.75%. Women with a history of abortion (38%), premature birth (30%), multiple sexual partners, or previous STIs were more likely to test positive . Lower education and delayed antenatal care initiation were significantly associated with syphilis positivity. Conclusion: While prevalence in Al-Nuhod locality was lower than other settings in Sudan, key risk factors persist. Early ANC attendance, routine screening, and targeted health education remain critical to reducing infection.