The banking system in India has evolved over several decades and is well established, serving the credit and banking needs of the economy. Poor working environments, including frequent work changes, overwork, role ambiguity, lack of clarity in communication, inappropriate leadership styles, lack of social support, transfers, and other factors, contribute significantly to the stress experienced by female bank employees. The main objective of the study is to explore the factors influencing job stress among women employees working in private banks in Bangalore city. Women employees from private sector banks such as ICICI Bank, HDFC Bank, Axis Bank, Kotak Mahindra Bank, and Karnataka Bank Limited were selected as the target population for data collection. The Yamane sampling method was used, and a total of 400 samples were analyzed. Linear regression analysis was employed using SPSS software for data analysis. Both primary and secondary data sources were utilized to collect the required information. The study revealed that both personal and organizational factors significantly influence the stress levels of women employees working in private sector banks in Bangalore city.