stdClass Object ( [id] => 15936 [paper_index] => 202505-01-021493 [title] => WHOLE CELL IMMOBILIZATION BY ENTRAPMENT METHOD FOR LIPASE PRODUCTION [description] => [author] => Mansi Pandav, Priyanshi Kanani, Sanjay N. Parekh [googlescholar] => [doi] => https://doi.org/10.36713/epra21493 [year] => 2025 [month] => May [volume] => 11 [issue] => 5 [file] => fm/jpanel/upload/2025/May/202505-01-021493.pdf [abstract] => Enzyme immobilization using biopolymers like sodium alginate enhances their stability and reusability, making them valuable in biotechnology. Soil samples from oil-contaminated sites in Surat, India, were screened on Tween 80-supplemented nutrient agar. A potent lipase producer, VC 13, was selected and characterized by morphological, biochemical, and molecularly characteristics and identified Cytobacillus sp. And its phylogenetic analysis was carried out. Whole-cell immobilization was done using 1.5% sodium alginate, forming CaCl₂-stabilized beads. Parameters such as temperature, pH, biomass, alginate concentration, and storage stability were optimized. Immobilized cells showed good enzyme activity, with optimal performance at 37°C, pH 8.5, 100 mg biomass, and 3% alginate. The enzyme retained activity over extended storage, suggesting its potential for industrial use. [keywords] => [doj] => 2025-05-11 [hit] => [status] => [award_status] => P [orderr] => 57 [journal_id] => 1 [googlesearch_link] => [edit_on] => [is_status] => 1 [journalname] => EPRA International Journal of Multidisciplinary Research (IJMR) [short_code] => IJMR [eissn] => 2455-3662 (Online) [pissn] => - -- [home_page_wrapper] => images/products_image/11.IJMR.png ) Error fetching PDF file.