stdClass Object
(
    [id] => 17160
    [paper_index] => 202507-01-023456
    [title] => SUB-CULTURING AND CRYOPRESERVATION OF MICROBIAL CULTURES: TECHNIQUES AND APPLICATIONS
    [description] => 
    [author] => Abu Shama, Dr.Raj Narayan Yadav, Kashif Raza 
    [googlescholar] => 
    [doi] => 
    [year] => 2025
    [month] => July
    [volume] => 11
    [issue] => 7
    [file] => fm/jpanel/upload/2025/July/202507-01-023456.pdf
    [abstract] => Microorganisms are fundamental tools in biological research and biotechnology and it's Applications to maintain their viability and genetic stability,More efficient preservation techniques are essential for this sub culture and cryopreservation of microbial Culture.
This paper reviews two fundamental methods—(a)sub-culturing (b)cryopreservation—focusing on their methodologies, advantages, limitations, and applications. Sub-culturing is a widely practiced short-term method for routine laboratory use, whereas cryopreservation serves as a long-term strategy to minimize genetic drift and maintain microbial stocks. Understanding the principles and protocols of both methods is crucial for microbiologists, biotechnologists, and researchers handling microbial resources.The preservation of this biological material has achieved the safeguarding of industrial potential and the possibility of researching new functions and the use for the benefit of humanity. Various conservation and preservation methods have been developed over the years, such as: sterile distilled water, cryopreservation, freeze-drying, sub-culture, and sterile mineral oil, the use depending on the microorganism and the desired viability and preservation time for long periods.The most used conservation methods are cryopreservation and freeze-drying, guaranteeing the stability of the characteristics of the strain (their viability, their vitality, and their biological potential) over time; however, the need to continue evaluating different methods.

    [keywords] => Sub-Culturing, Cryopreservation,Microbial Strain,Phenotypic and Genotypicstrain, Microorganisms , Fungus,Biotechnology.
    [doj] => 2025-07-29
    [hit] => 
    [status] => 
    [award_status] => P
    [orderr] => 132
    [journal_id] => 1
    [googlesearch_link] => 
    [edit_on] => 
    [is_status] => 1
    [journalname] => EPRA International Journal of Multidisciplinary Research (IJMR)
    [short_code] => IJMR
    [eissn] =>  2455-3662 (Online)
    [pissn] => - --
    [home_page_wrapper] => images/products_image/11.IJMR.png
)
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