This study investigates the relationship between the level of education and syphilis knowledge among pregnant women. This research emphatically highlights the role of educational level as a critical determinant of syphilis knowledge and its transmission routes among pregnant women A descriptive cross-sectional facility-based study was conducted using total coverage sampling of 400 pregnant women attending antenatal care (ANC) centers in Al Nuhod Town, West Kurdofan State, Sudan, from 2018 to 2021. Data were collected via interview, a structured questionnaire, and laboratory examination for Syphilis. The analysis utilized the Statistical Package for Social Science (SPSS) program. The overall prevalence of Syphilis was found to be 0.75%. A total of 73.3% of participants had attained a high level of education (secondary or university degree);however, general knowledge about syphilis was still limited, with 78.3% reporting no knowledge of the disease. Study provides conclusive evidence(P-value = 0.000) of a strong,statistically significant correlation between higher educational attainment and greater syphilis knowledge.this confirmation is crucial as the study was conducted in Al-Nuhod,an area previously reported to have the highest syphilis prevalence(6.28%) among pregnant women in Sudan. Knowledge among illiterate participants was notably lower (3.9% reporting "Yes" to syphilis knowledge) compared to those with a university education (27.9% reporting "Yes"). The findings underscore that education plays a major role in reducing syphilis incidence. The study recommends the urgent planning and implementation of effective health education programs to increase awareness of syphilis and its transmission routes within the community.