Women entrepreneurship plays a vital role in promoting economic growth, employment generation, and social development. However, women entrepreneurs often experience significant levels of stress due to financial constraints, market competition, work–life imbalance, family responsibilities, and operational challenges associated with managing businesses. In the context of emerging urban business environments such as Tumakuru City, understanding stress management practices among women entrepreneurs has become increasingly important for ensuring sustainable entrepreneurial development and personal well-being. The present study titled “A Comprehensive Study on Stress Management Practices among Women Entrepreneurs in Tumakuru City with Special Reference to Entrepreneurial Opportunities and Business Challenges” aims to examine the major stress factors affecting women entrepreneurs, analyze the stress management techniques adopted by them, and evaluate the influence of entrepreneurial opportunities and business challenges on their stress levels and business performance. The study adopts a descriptive research design and is based on both primary and secondary data. Primary data were collected through a structured questionnaire administered to women entrepreneurs engaged in various business activities in Tumakuru City. Appropriate statistical tools such as percentage analysis, mean analysis, correlation, and chi-square test were applied for data interpretation.