Environmental DNA (eDNA) is a rapidly emerging method that enables the detection of species and their genetic material in environmental samples like water, soil, or air, without the need to physically capture or observe the species. This technique has revolutionized conservation biology, offering an efficient, non-invasive, and cost-effective means of monitoring biodiversity and ecosystem health. By isolating DNA from various environmental samples, eDNA can identify species present in an area, track population dynamics, assess species distribution, and even detect invasive species. This paper explores the applications of eDNA in conservation, highlighting its role in biodiversity monitoring, species conservation, and ecosystem management. Through case studies and ongoing research, the paper demonstrates the potential of eDNA to address critical conservation challenges, such as monitoring endangered species, controlling invasive species, and studying biodiversity in remote or difficult-to-access ecosystems. The paper also discusses the limitations and challenges of using eDNA, including issues related to data interpretation, standardization, and the need for further validation in different ecosystems.