The purpose of the study was to find out the prediction of selected anthropometric, physical and physiological variables with playing ability of district women handball players. 150 state level handball players were participated as the samples for the study. Different variables namely (Weight, Standing height, Sitting height, Arm length, Arm span, Palm breadth, Chest girth, Waist girth, Hip girth, Thigh girth, Calf girth, Speed, Agility, Flexibility, Leg explosive strength, Muscular endurance, Harvard step up, Resting heart rate and Peak expiratory flow rate) were measured of the samples. Standard procedure was followed to measure the anthropometric, physical fitness and physiological variables. To measure the playing ability, five game experts were asked to rate the players playing ability for ten marks. After that all five experts’ marks were added and averaged to get the marks of playing performance. Karl-Pearson’s co-efficient of correlation technique was used to find out the relationship between anthropometric, physical and physiological measurement and playing performance. Very few anthropometric measurements found significant with skill performance. The implications of results are discussed.