Present study is carried out to evaluate the analgesic activity of given drug Vitex nigundo Linn on mice. The fresh leaves of Vitex nigundo Linn. have been suggested to possess analgesic activity possibly mediated via prostaglandin (PG) synthesis inhibition Because it contain alkaloids, glycosides, flavonoids sterols, resin and tannins as per phytochemical evaluation of extract The Hot plate test used in this experiment to study the antinociceptive activity of ethanolic leaf extract of Vitex-negundo (VN) (500 mg/kg, p.o) on mice. The effect was compared with Diclofenac sodium (25 mg/kg, i.p) in Hot plate test & standard &control respectively. Vitex nigundo Linn has a mechanism of central analgesic action. The test drug showed significant analgesic activity in dose dependent manner in the experimental models. Our observations suggest Vitex nigundo Linn that possesses both central peripheral analgesic activities .It may prove to be a useful as analgesic drug.