Thiosemiccarbozones are having enormous medicine applications due to the presence of hetero atom nitrogen and sulfur in its molecular structure. The carbonyl compounds are also having the largest clinical applications to cure many diseases. We merged these two classes of organic compounds and their substitution by solution growth techniques of crystal growth tested as antibiotic against few fastidious and non fastidious gram +ve and gram –ve organisms. Ortho substituted para substituted and Meta substituted benzaldehydes were merged with thiosemicarbazone and prepared. Thiosemicarbazone of (2-chlorophenyl) methylideneamino thiourea, when compared with linear optical material, these NLO have high medicinal application since it is the photo dynamic therapeutically more active, proved by the early researches mentioned in the experimental part. These one compounds were tested against. Streptococcus Aureus, the following experimental method was adopted find out the antimicrobial activity. MTT assay by colorimetric method, Cell viability percentage calculation method, Half inhibitory maximum (IC50) by calculation method. Agar disc diffusion method. Inhibition zone width by agar disc diffusion method, EUCAST and NCCLS database analysis to compare the MIC and inhibition zone width of existing antibiotic against the above said one organism, Graphpad prism software is used to find out the absolute, relative IC50 value and hill slope value, ECOF finder software to find out the epidemiology cutoff value, WHONET 5.6 software analysis is used to find out resistant, susceptible or intermediate nature of commercially existing antibiotics. Size and structure of the newly designed antibiotics compare with the structure of commercially existing antibiotics and structure of organisms.