This study was designed to investigate the impacts of ladder training on selected physical fitness variables among intercollegiate women kabaddi players. Thirty women kabaddi players were randomly selected from Dr. Sivanthi Aditanar College of Engineering, Tiruchendur, Govindammal Aditanar College for women, Tiruchendur, Wavoo Wajeeha womens college of arts and science, veerapandipattanam were participant selected. The age of the participant was ranged from 18 to 25 years. They were divided into two equal groups. The group 1 is considered as experimental group (ladder training) and group 2 was considered as control group. Pre-test was conducted on Speed, Agility and leg explosive power for both the groups and the reading were carefully recorded in their respective unit as pre-test score. After pre test, experimental group was treated with specific ladder training, for duration of 60 minutes, three days per week for a period of twelve weeks. The control group was not treated with any special training. After twelve weeks of training post test was conducted and the reading were carefully recorded in their respective units as post test score. The pre and post test were taken for analysis. The collected data on physical fitness variables due to twelve weeks ladder training was analysed by dependent t test with 0.05 level of confidences. From the results of the study, it was found that there was a significant improvement on physical fitness variables among intercollegiate women kabaddi players.