This article shows that the relevance of the method of microclonal propagation and cultivation of plants under in vitro condition as a cost-effective technology for the production of planting materials that allows growing plants in sufficient quantities for scientific and production purposes has been substantiated. By determining the efficiency of new generation growth stimulants, which were not previously used in intensive in vitro culture, and comparing them with the standard growth substances used in clonal micropropagation, an economic evaluation of the technology of cultivating cherry rootstocks in intensive in vitro conditions, developed in the laboratory Biotechnology of the scientific research institute of horticulture, viticulture and winemaking named after Academician Mahmud Mirzayev and its efficiency was carried out. The economic appraisal of the technology of reproduction of promising cherry rootstocks in intensive in vitro conditions includes the selection of the object for the introduction of innovations, the description of the technology, the calculation of costs and the determination of economic evaluation indicators.