Ayurveda the natural healing system guides us the use of different kinds of herbal, mineral and animal origin drugs. The quality control and assurance are most essential factor in todays practice. Hence the present study has been taken to analyze Amrutakalaanidhi Vati (AKN Vati) as per Ayurveda classics and modern parameters like physicochemical tests, XRD, SEM EDAX. AIMS AND OBJECTIVES: To do Pharmaceutico-Analytical study of AKN Vati. Materials and Methods: Preparation of AKN vati and physic-chemical analysis of AKN vati and Varatika at different stages. RESULTS: Preparation yielded a good quality of vati and the overall methods adopted were not too tedious. XRD showed change in crystallinity of calcium compound and SEM-EDX showed calcium and silica as major elements in VB and AKN Vati. Discussion and Conclusion: AKN vati indicated in Jwara and Agnimandya. To gain acceptance of the medicine at the global level present study focused on pharmaceutical and analytical aspects.