Integrating physical education and health curriculum has become a prevalent trend, aiming to promote students overall well-being. This research investigates the interdisciplinary cooperative teaching practices of physical education teachers in this integrated curriculum context, exploring the application of health assessment tools and methods and the formulation of individualized exercise plans. Utilizing a mixed-methods approach, the study combines qualitative and quantitative data collection and analysis techniques involving physical education teachers, health professionals, and students from selected schools. The findings reveal the current status of curriculum integration, highlighting challenges and opportunities, and delve into the utilization of health assessment tools for physical fitness, physiological health, and psychological well-being evaluations. Case studies and best practices elucidate the process of developing individualized exercise plans based on these comprehensive health assessments. The research proposes strategies for effective interdisciplinary cooperative teaching, offers recommendations for the appropriate selection and application of health assessment tools and personalized exercise plans, and discusses limitations and future research directions. This study contributes to the growing knowledge of curriculum integration and interdisciplinary collaboration in physical and health education, informing educational policymakers, curriculum designers, and practitioners in promoting holistic student development.