The objective of this study was to find out the impact of functional interval training on physiological variables of female volleyball players. To achieve the purpose of this study, forty female volleyball players were selected SRM Institute of Science and Technology, Kattangulathur, Chengalpattu. Their age was ranged from 14 to 17 years. The selected subjects were randomly divided into two groups such as Group ‘I’ underwent functional interval training (n=20) and Group ‘II’ acted as control group (n=20). Group ‘I’ underwent functional interval training for three alternative days and one session per day and each session lasted for an hour for eight weeks period. Group ‘II’ was not exposed to any specific training but they were participated in regular activities. The data on and vo2 max was measured by cooper vo2 max test (ml/kg), breath holding time was measure by breath holding test (seconds) and resting pulse rate was measured by bio monitor (seconds). The pre and post tests data were collected on selected criterion variables prior to and immediately after the training programme. The pre and post tests scores were statistically examined by the dependent‘t’ test. The level of significance was fixed at 0.05 levels for all the cases in order to find out the significance. It was concluded that the functional interval training on vo2 max, breath holding time and resting pulse rate variables among female volleyball plyers. However the control group had not shown any significant improvement on selected variables such as vo2 max, breath holding time and resting pulse rate.