The purpose of this study was to find out the effect of interval training on skill related physical fitness variables among Kho-Kho players. To achieve the purpose of the present study, thirty Kho-Kho players were selected as subjects at random and their ages ranged from 21 to 25 years. The subjects were divided into two equal groups. The subjects (n=30) were randomly assigned to two equal groups of fifteen kho-kho players each. The interval training group participated the training for a period of eight weeks and the post-tests were conducted. The subjects were tested prior to and after the experimentation on Reaction Time, Cardio Vascular endurance and agility. Dependent ‘t’ test was used to test the treatment effect of the training programmes on all the variables used in the study. It was observed that the eight weeks of interval training have significantly improved the selected skill related physical variables of kho-kho players.