Burn injuries continue to be a significant global health concern, often resulting in severe morbidity and mortality. Traditional herbal remedies have long been utilized for wound healing, with Jatropha curcas emerging as a promising candidate due to its reported anti-inflammatory, antimicrobial, and wound healing properties. In this study, we aimed to formulate and evaluate herbal burn healing cream using Jatropha curcas latex. The cream formulation was developed using a combination of Jatropha curcas latex extract, along with compatible excipients to optimize stability, texture, and therapeutic efficacy. The formulated cream was subjected to various physicochemical, rheological, and pharmacological evaluations to assess its suitability for burn wound management. Physicochemical analysis revealed the cream to be stable with desirable pH, viscosity, spreadability, and consistency. Rheological studies demonstrated indicating ease of application and spreadability on the skin. Furthermore, in vitro antimicrobial assays exhibited significant inhibition of common wound pathogens by the formulated cream. It has been reported that this latex has several biological activities such as antibacterial, antioxidant, anti-inflammatory, and wound healing.