The aim of present investigation is one of traditionally used herbs anti-Hepatotoxicity being assessed against the Paracetamol induced Hepatotoxicity in rats.The drug related Hepatotoxicity is uncommon for many drugs, the reported incidence is between 1 in 10,000- its true incidence is difficult to determine. The number may be much higher,because of underreporting,difficulties in detection or diagnosis,and incomplete observation of persons exposed (Navarro and John, 2006). Paracetamol treatment caused significant (p<0.001) decreases in the activities of SOD, catalase, GPx and GSH level in liver tissue when compared to control group.. Silymarin-treated animals also showed a significant (p<0.001) increase in antioxidant enzymes, namely SOD, catalase, GPx activities and GSH level compared to paracetamol-treated rats.To understand the effect of the extract on liver, histology of liver was performed. Firstly, Organoleptic Characterization of plant extract was performed. Organoleptic evaluations are subjective, sensory judgements. They can involve eyeing, feeling and taste of the extract to the judge its appearance, coour, integrity, texture and flavours. The organoleptic characters of alcoholic extract of Cnidoscolus Quercifolias leaf extract were found to be dark green in colour, semi solid, and taste is acrid. Solubility testing of alcoholic extract of Cnidoscolus Quercifolias leaf is done mainly to study the ability of the dissolve in different solvent for the preparation of aqueous extract for dosing. The alcoholic extractwas observed to be dissolved in water and DMSO. In the present study, the preliminary phytochemicals test was done on the alcoholic extract of Cnidoscolus Quercifolias leaf and it is found to be rich in Carbohydrates, Proteins, Saponin, Flavonoids and Phenolic compound. For the determination of protective effect of Cnidoscolus Quercifolias leaf extract against paracetamol induced Hepatotoxicity, firstly level of GSH and SOD was checked. GSH and SOD level was tested in vehicle treated group after that Paracetamol treated group and then 200 mg/kg of plant extract along with Paracetamol treated group and after that 400 mg/kg of plant extract along with Paracetamol treated group