A herbal handwash was prepared using extracts of leaves of curry leaves, hibiscus powder, turmeric powder, reethe, orange pill, potato pill, tabrind pulp. The antibacterial of the prepared herbal handwash against skin pathogens was checked using Disc diffusion method and results were compared with the commercially available antiseptic soap. The results showed that the herbal handwash gave larger inhibition zone than the commercial antiseptic soap against Staphylococcus aureus and Pseudomonas aeruginosa. The efficacy of the herbal handwash was evaluated using Glove Juice method which revealed that the herbal handwash is efficient in reducing higher number of organisms from the hands than the commercial antiseptic soap. Hence, due to the higher antimicrobial activity these plant materials can be used in the preparation of herbal handwash on a commercial scale.