Estimation of Quinine Sulphate Using a Photo Fluorometer Quinine sulphate, a widely used antimalarial drug, can be accurately quantified using photo fluorometry due to its inherent fluorescence properties. This study aims to develop and validate a sensitive, precise, and reproducible method for the estimation of quinine sulphate in pharmaceutical formulations. The procedure involves preparing standard solutions of quinine sulphate and measuring their fluorescence intensity at an excitation wavelength of 350 nm and an emission wavelength of 450 nm. The calibration curve, constructed by plotting fluorescence intensity against quinine sulphate concentration, demonstrates linearity within the range of 0.1 to 10 µg/mL, with a correlation coefficient (R²) greater than 0.99. The method exhibits high sensitivity, with a detection limit of 0.05 µg/mL and a quantification limit of 0.1 µg/mL.