In view of the need for a suitable Spectroscopic method for routine analysis of Tenofovir(antiretroviral medicine) in formulations, attempts were made to develop simple, precise and accurate analytical method for estimation of Tenofovir in formulation. As Validation is a necessary and important step in both framing and documenting the capabilities of the developed method it was done in accordance with USP and ICH guideline for the assay of active ingredient. The method was validated for parameters like linearity, precision, accuracy, specificity, and robustness, limit of detection and limit of quantification. This method provides means to quantify the component. This proposed method was suitable for the analysis of Pharmaceutical dosage forms.The utility of the developed method to determine the content of drug in commercial formulation was also demonstrated.