In an attempt to strengthen the scientific foundation for the extraction of bioactive components from the plant, the cytotoxic and antioxidant properties of Croton bonplandianum Baill's methanolic and dichloromethane extracts were assessed. Dichloromethane extract was not poisonous, and the methanolic extract had 59.62% DPPH and hydroxyl radical scavenging activity. The reference compound, gallic acid, displayed greater antioxidant activity than the plant extracts. The lethality of both methanolic and dichloromethane extracts on brine shrimp was assessed. An in vitro cytotoxic activity test for methanolic extract yielded an LD50 value of 115.76 (0.0048 - 13.76) µg/ml. The standard medication used was etoposide.The standard medication used was etoposide. Together, these findings support the notion that Croton bonplandianum extracts have significant inherent cytotoxic and antioxidant potentials, which is a strong argument in favor of isolating pure bioactive substances. The plant's parts are widely used in traditional medicine to treat a wide range of conditions, including wound healing, hepatoprotective, body swelling, hypertensive, abdominal dropsy, anti-fungal, anti-microbial, antidiabetic, anti-tumor, and anti-cancer. Because traditional multiplication proceeds slowly, the plant is abundant. Its traditional medical applications have been proven by several in vivo and in vitro tests.