The main objective of present work is formulation and evaluation of multiple emulsion of clotrimazole. Multiple emulsions dosage formulation of clotrimazole which has enhanced release and bioavailability properties with less inter and intra- subject variability would be desirable. Thus, it was aimed to formulate and evaluate the multiple emulsion of clotrimazole. By the preformulation studies it is observed that clotrimazole is a white to pale yellow crystals having no odor. Solubility was determined in various solvents found that freely soluble in ethanol and methanol, slightly soluble in Distilled Water, soluble in Phosphate Buffer 6.8 pH,0.1N HCl and 0.1N NaOH. Melting point was observed in range of 147-149 0C. Partition coefficient was 1.797 obtained. Drug: Excipient Compatibility Studies at room temperature, 20C-80C and 450C-500C says it is stable. Stability also confirmed by FT-IR studies. Five different type formulations (ME-1 to ME-5) formed using fixed amount of oil phase. All five Clotrimazole multiple emulsion formulations were odorless, washable, homogeneous, stable and free from grittiness and was evaluated under the various parameters, Ph of all formulations were observed at 6.8 and viscosity between 4 0.23 to 76.38 centi poise and percentage of Drug content between 83.31 to 95.23%. In-vitro Drug Release of Clotrimazole multiple emulsion formulations were studied and found ME-1 (87.61), ME-2 (94.18), ME-3 (81.74), ME-4 (83.74) and ME-5 (78.62). Formulation ME-2 was found excellent on the basis of cumulative percentage of drug release profile. All five formulations were also tested for stability and found formulation ME-2 was stable after 30 days against color change, creaming, creaking and phase separation. Optimized formulation (ME-2) drug release data were fitted into the zero order, first order, Higuchi and Peppas-Korsemeyer model of drug release kinetics. Formulation ME-2 was followed Zero Order Kinetics explained as continuous and steady release of Clotrimazole from the formulation.