Nowadays HPLC is widely used instrumentation technique for separation and purification in various areas such as Biotechnology, Pharmaceuticals, Environmental food and polymer Industries.In this Review of High- Performance liquid chromatography contain Introduction, Chromatographic terms, Types and different classes of HPLC . As well as contain brief introduction to HPLC principle and Instrumentation, pumps, Included in detail with their efficiency. This article is Written with the intension of reviewing several HPLC related topics including instrumentation , principles , manner of separation, characteristics, key parameter and Numerous application in various fields