Since many centuries, Ayurveda regarded neem (Azadirachta indica Family: Meliaceae) as a cure for many ail-ments, predominantly due to its superb antimicrobial activity. It has been a practice since time immemorial to use tender twigs of neem as dentifrice. Looking as these facts the possibility of developing an authentic dentifrice from neem extract and formulations were formulated and evaluated for their antimicrobial activity and some formulations were found to have significant antimicrobial activity.The main objective of this review article is to compile the available information related to herbal toothpaste like its introduction, different formulations and different parameters on which these herbal toothpastes can be evaluated. This information can be used by many researcher who wants to make research in this area.These herbal toothpastes can be evaluated by different tests like Physical Examination, Relative density, Abrasiveness, Determination of spreadability, pH determination, Homogeneity, Foaming,Stability, Determination of moisture and volatile matter, Moisture content, Foaming character, Organoleptic evaluation, pH, Fragrance test, Shape retention, Storage stability, Total flavonoid content estimation of Tooth paste Formulation, Stability study (Storage stability), Antimicrobial activity of toothpaste etc.