A total of 294 single spores were isolated from eleven-button mushroom strain and 132 were evaluated for their fertility of which 24 were found non-fruiting whereas eleven single spore isolates started fruiting after second flush of cropping and remaining were fertile. button mushrooms (Agaricus bisporus) were coated by dissolving four different organic coating material viz. apple peel powder), carboxymethyl cellulose, tartaric acid, and glycerol monostearate in distilled water and dipping the mushroom in coating solution for 45 to 75 experiments were conducted using response surface methodology and the coated as well as uncoated samples were stored at ambient and refrigerated conditions. Various quality parameters of button mushroom were measured. During the last decade several major breakthroughs have been achieved in mushroom biotechnology, which greatly enhanced classical mushroom breeding. Other hand efficacy of developed organic coating powder tested on button mushroom (Agaricus bisporus) shows some prominent practical application for fresh produce. From analysis and interpretation of the experimental result it was found that organic coating powder was effectively increase the shelf life of button mushroom also maintained its quality.