Business development drives economic growth and innovation, influenced by macroeconomic stability, regulatory quality, and financial access. This study analyzes Uzbekistan’s business environment (2010–2022) using statistical evaluation of key factors affecting the Ease of Doing Business (EDB) score. Results show that regulatory quality, government effectiveness, GDP per capita, and FDI positively impact business growth, while inflation has a negative effect. Findings highlight the need for policy reforms, financial support, digital transformation, and human capital investment to foster a competitive business ecosystem.