The aim of the present study is to evaluate the anti-inflammatory, anti-microbial and anti-fungal activity of Murraya koenigii leaves. The male wistar rats having weight (150-200g) were used. The petroleum Ether, chloroform and ethanol extracts were prepared by using soxhlet extraction method. The petroleum ether, chloroform and ethanol extracts of Murraya koenigii leafs were screened at dose of (250 mg/kg) for anti-inflammatory activity by using acute carrageen induced paw oedema method and yeast induces hyperpyrexia method respectively. The ethanolic extract shows significant effects in anti-inflammatory activity. This study had rationalized the ethanomedicinal use of the plant for cut, injury & alignment of body temperature by treble people. The Methanolic Extract of Murraya Koenigii Leaves (curry leaves), were investigated against different spices of Gram –ve bacteria i.e. Pseudomonas aeruginosa, Escherichia Coli, Klebsiella and Gram +ve Staphylococcus aureus and fungus Candida albicans in present study. The screening was performed by standard disc diffusion method. It is demonstrated moderate antibacterial activity against Staphylococcus aureus having the diameter of zone of inhibition of 15 mm and good antibacterial activity against E. coli with zone of inhibition of 20 mm and Pseudomonas aeruginosa with zone of inhibition of 10 mm and no zone of inhibition on Klebsiella, The leaf extract has shown anti-fungal activity on Candida albicans with16mm zone of inhibition.