The rapid evolution of technology has ushered in a new era in IT sector marked by distinct phases known as industry 4.0 and Industry 5.0. The integration of cyber physical systems, IoT, AI, enabling enhanced automation and efficiency. This paper explores the curial role of knowledge transfer in facilitating this transition from Industry 4.0 to Industry 5.0. it not only the adoption of new technologies but also the transfer of knowledge, skill and culture across generations of workers and industries. It also discusses the challenges and opportunities of knowledge transfer. The data has been collected by the questionnaire among the employees and some of the journal etc., the researcher analyses the relationship between with correlation, impact with regression. The major finding of the study is organizations need to continually update their skills and processes to keep pace with rapidly advancing technology and it also indicates that effective knowledge transfer between humans and AI driven systems was essential for optimizing productivity and decision making