Background: In Ayurveda, Dosha, Dhatu, and Mala form the foundational elements of the human body, with Vata Dosha being a key regulator of physiological movements and functions. An imbalance in Vata leads to various health disorders, emphasizing the need for its accurate assessment in clinical practice. Aims and Objectives: To analyse and evaluate the Karma of Vata Dosha through both subjective and objective parameters, aiming to enhance diagnostic accuracy and treatment. Materials and Methods: Classical Ayurvedic texts such as Brihattrayi and Laghutrayi were referred to for understanding Prakrita and Vaikrta functions of Vata. Relevant contemporary medical literature was reviewed to correlate traditional concepts with quantifiable physiological indicators. Discussion and Conclusion: Subjective parameters include clinical history, Prakriti and Vikriti analysis, and sensory observations, while objective parameters involve clinical tests and diagnostic tools assessing sensory, motor, and cognitive functions. Prakrita Karma like Utsaha, Uchchvāsa, and Cheshta reflect normal Vata activity, whereas Vaikrta Karma due to Vata Vṛddhi or Kṣhaya manifest as conditions such as Karshya, Kampa, Anaha, Nidra Bhramsha, and Agni Sādana, which can be evaluated through structured clinical methods. Integrating both approaches allows for a more comprehensive understanding of Vata Dosha Karma, supporting evidence-based diagnostics and individualized therapeutic strategies in Ayurvedic practice.