The present study is an attempt to primarily focuses on the trends and levels of awareness and comprehensive knowledge of HIV/AIDS among men and women in Arunachal Pradesh. Taking the various reports of National Family Health Survey Data of various round, the present study shows that the awareness of HIV/AIDS and knowledge of prevention and transmission differs based on gender, religion, caste, education level, location and age group. The study reveals that the knowledge of HIV/AIDS among both men and women in Arunachal Pradesh is increasing from NFHS-3 to NFHS-5. Although men have more knowledge of HIV/AIDS than women, both genders show improvement over time. The comprehensive knowledge of HIV/AIDS remains low, especially among women, despite overall increases in awareness and recognition of preventive measure like use of condom.