Hand sanitizer is an alternative to the hand washing with soap water. Hand sanitizer is the most important measure to avoid the transmission harmful microbes and prevent the infection, to keep the skin safe from harmful microorganism and to prevent spreading of many infectious diseases. The crucial I deal and practice in the prevention, management and decrease of diseases is hand cleanliness. We created aloe vera hand sanitizer utilizing other components such Eucalyptus oil, hydrogen peroxide, glycerine, vit E, Ethanol, distilled water, camphor. Different formulation are prepared and characterised using eucalyptus oil is an active pharmaceutical ingredient. The PH of herbal sanitizer found to be 6-6.5 with no irritation to skin. Natural aloe vera hand sanitizer are affordable, effective and environmentally friendly.