The present study aimed to examine the relationship between fear of failure and playing ability among women Kho-Kho players. A total of 120 players who reached the quarterfinal stage of the South Zone Inter-University Kho-Kho tournament were selected, with ages ranging from 18 to 25 years. Fear of failure was measured using the Performance Failure Appraisal Inventory (PFAI), which includes five dimensions along with an overall fear score. Playing ability was evaluated through subjective ratings on a 10-point scale based on selected skill performance variables (Pole Turn, Pole Dive, 3-3-2 Up, Diagonal Attack, Judgement Kho) and tactical performance variables (Clubbing and Avoiding). The collected data were analyzed using Karl Pearson’s Product Moment Correlation. The results showed that all dimensions of fear of failure were significantly and positively related, with stronger associations observed for fear of upsetting important others and fear of uncertain future. Similarly, all skill performance and tactical performance variables were significantly interrelated, indicating that improvement in one skill is associated with improvement in others. The findings highlight that psychological factors and playing abilities are closely connected, and reducing fear of failure may help improve performance in Kho-Kho players.