This article covers the formation, development and prospects of cultural and humanitarian cooperation between the Republic of Uzbekistan and Central Asian countries. Factors influencing the cultural and humanitarian cooperation of Uzbekistan with the countries of the region are analyzed. The role and place of the cultural and humanitarian sphere in strengthening cooperation between the countries of Central Asia is gradually shown. The legal basis of interstate cultural and humanitarian cooperation and the activities of cultural events are studied. The common history and spiritual values of Uzbekistan, Kazakhstan, Kyrgyzstan, Tajikistan and Turkmenistan are illustrated by examples. It has been revealed that the ancient fraternal ties between the peoples of the region today serve to strengthen interstate cultural and humanitarian cooperation. Conclusions were reached on the development of cultural and humanitarian cooperation between Uzbekistan and Central Asian countries, and a number of proposals were made to strengthen cultural cooperation.