Introduction: Menstruation is a periodic cycle which occurs after every 28-30 days in every healthy woman after menarche till menopause. In recent years, this blood is considered as best out of waste as pluripotent stem cells can be collected from it. In addition to this, it could be saved for future benefits. In this study, an attempt has been made to rule out nursing student’s cognizance regarding menstrual blood banking. Aim of the study: To assess the knowledge regarding menstrual blood banking among nursing students at Jodhpur. Material and Method: A descriptive study was carried out on 180 nursing students of Government Nursing College, Jodhpur. Non-probability purposive sampling method was used to select samples. Study was conducted from 17th September to 17th October’2020 using structured knowledge questionnaire. Moreover, study was analysed by using descriptive and inferential statistics. Result: The findings of the study reveals that majority (58.03%) of the nursing students had moderate knowledge regarding menstrual blood banking. Furthermore, the study illustrated a significant association between level of knowledge and selected socio- demographic variables. Conclusion: It can be reiterated that nursing students have an average knowledge regarding menstrual blood banking.