Mulberry leaf is a major economic component in sericulture since the quality and quantity of leaf produced per unit area has a direct bearing on cocoon harvest, although various highly productive mulberry varieties and appropriate cultivation packages have been developed to produce a high quantum of quality leaf. Data recorded on Bio-chemical analysis viz. Total Soluble Carbohydrate (TSC) (mg/g dwt) Total Soluble Protein (TSP)(mg/g dwt) and Total Chlorophyll Content (TCC) (mg/g dwt) at all 03 sites and Bioassay studies show that 02 mulberry varieties i.e. S-140 (PPR-1) and Tr-10 were found superior to other varieties including S-146, which was taken as control under this experiment.