The field experiment was carried out about defining the influence of organic, mineral fertilizers and biological preparations on the growth and development of cotton in saline soil conditions of the Republic of Karakalpakstan. On the basis of the results of the research it was determined that using 40 t/ha organic fertilizer, decreasing the amount of mineral fertilizer to 25% and using in the amount of N125 P90 K60 and from biological preparations using 2 l/ha of phytovar, influence positively on the growth and development of cotton. In this, the growth and development of cotton was higher than other variants.