INTRODUCTION: Children are the world’s most valuable assets and their wellbeing indicates the standard of living of the country. They constitute one third of the total population. It has been noticed that almost one out of every five live born infants die before reaching 5 years of age. Immunisation is one of the most important weapons for protecting individuals and the community from serious diseases. The Department’s immunisation team supports the public and health professionals by offering clear, evidence-based information about different diseases, the possible side effects of immunization and available vaccines. AIM OF THE STUDY: Assess the knowledge regarding vaccine preventable disease among mothers of under five children. METERIAL AND METHOD: A descriptive study was carried out to assess the knowledge of 60 mothers selected by purposive sampling, were assessed by using a structured knowledge questionnaire and analyzed by using descriptive and inferential statistics were used for data analysis. RESULT: The findings of the study reveals that to the level of knowledge shows that majority of 60 mothers of under five children in which (33) 55% had below average knowledge, (18) 30% had average knowledge and (9) 15% of them had above average knowledge regarding vaccine preventable diseases. CONCLUSION: It can be concluded that mothers have below average knowledge regarding vaccine preventable diseases as per current research recommendations. They require education and to enhance their knowledge regarding vaccine preventable diseases. Self-information booklets were distributed to them to improve the knowledge regarding vaccine preventable diseases