The antimicrobial property of black seed was used in this formulation, the present work designed to form a topical herbal cream with safety and less side effects. This formulation contains black seed oil which contains active chemical components like thymoquinone, alkaloids, flavonoids, saponins, glycosides. Thymoquinone is the main chemical which shows the antimicrobial activity. The formulation contains Carbopol, steric acid, cetyl alcohol as an emulsifying and thickening agent. The formulation was studied for various evaluation parameters like pH, viscosity, spreadability, type of emulsion, particle size, drug content, antimicrobial test. By studying all the parameters from all the formulation, the batch no F4 and F5 formulation shows good result.