INTRODUCTION: A chest tube is a hollow, flexible tube placed into the chest. It acts as a drain. Chest tubes drain blood, fluid, or air from around the lungs, heart, or oesophagus. The tube around the lung is placed between the ribs and into the space between the inner lining and the outer lining of the chest cavity or the pleural space. It is done to allow the lungs to fully expand. It is also known as bulau drain or an intercostal catheter. AIM OF THE STUDY: Assess the knowledge and practice regarding nursing management of patients with chest tube drainage among staff nurses working at tertiary care hospital, Jodhpur. MATERIAL AND METHOD: A descriptive study was carried out on 60 staff nurse working in ICU’s of M.D.M hospital, Jodhpur. Samples were selected by using non probability purposive sampling technique. Data was collected using structured knowledge questionnaire and practice checklist were analyzed by using descriptive and inferential statistics. RESULT: The findings of the study reveals that 55% ICU staff nurses had average knowledge, with mean knowledge score 14.47 with SD ± 4.68, while majority of ICU staff nurses (96.67%) had good level of practice, (3.33%) had average level of practice, with mean practice score 13.93 with SD +1.41 regarding nursing management of patients with chest tube drainage. It was found that there was no significant correlation found between level of knowledge and practice among ICU staff nurses regarding nursing management of patients with chest tube drainage. However there was no significant association found between level of knowledge and practice with selected socio demographic variables except their total clinical experience. CONCLUSION: It can be reiterated that ICU staff nurses have average knowledge and good level of practice regarding nursing management of patients with chest tube drainage as per current research recommendations. They require simulated practices, CNE and Role play to enhance their knowledge and practice regarding nursing management of patients with chest tube drainage.