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ANTIDIABETIC ACTIVITY OF POLYHERBAL FORMULATION IN DEXAMETHASONE INDUCED DIABETES IN WISTAR ALBINO RATS


G. Mahesh, Nimmala Shanthi, Nagamani, S. Jayanthimala
Department of Pharmaceutical Sciences, Vikas College of Pharmaceutical Sciences, Rayanigudem(v), Suryapet (Dist). T.S. India.
Abstract
Background: Diabetes mellitus (DM) is a group of disorders that results in too much sugar in the blood due to impairment of lipids, carbohydrates, proteins metabolism. Aim and objectives: Development and Evaluation of Polyherbal formulation (PHF) and determination of antidiabetic potential of developed formulation in Dexamethasone induced animal model. Method: In the present study plant parts Azadirechta indica (AI) leaves, Moringa Oleifera (MO) fruits and Andrographis paniculata (AP) root and stem were collected and evaluated as per physico-chemical parameters and active chemical constituents were extracted using hydroalcoholic solvent. The active compounds present in all the three extracts were identified by preliminary phytochemical screening. PHF was prepared in a ratio of 1:1:1 quality of the finished product was evaluated on the parameter’s angle of repose, loose bulk density, tapped bulk density, carr’s index and hausner ratio as per the World Health Organization’s (WHO) guidelines for the quality control of herbal materials. The acute toxicity study of PHF were performed as per OECD guideline 423, rats were orally administered 250, 500, 1000 and 2000mg/kg over 14 days. The oral glucose tolerance test (OGTT) was performed at 200 and 400mg/kg body weight. Antidiabetic activity of the PHF (200 and 400mg/kg) was screened against Dexamethasone (DXM) induced diabetes in rats and glibenclamide was used (5.0mg/kg body weight) as standard drug. The investigational drug was administered for 14 days and the effect of the PHF on blood glucose levels was studied at 14th day after interventional period. At the end of the study, the blood samples were collected from all the animals for biochemical estimation. Result: The plant parts AI leaves, MO fruits, AP stem and leaves were evaluated as per physicochemical parameters and they were found as per API. Preliminary phytochemical screening of hydroalcoholic extracts were revealed that presence of alkaloids, glycosides, saponins, flavonoids, carbohydrates, steroids, tannins and phenolic compounds in each extract. PHF were developed by mixing of each extract in the same ratio and evaluated. It was found to be angle of repose (θ) 29.1, loose bulk density 0.48gm/ml, tapped density 0.54gm/ml, carrs index 12.50%, hausner’s ratio 1.13. Diabetes was induced by DXM and treated with PHF did not show any change in behavior and no mortality was observed during interventional period upto the dose level 2000mg/kg. OGTT was performed by oral administration of PHF with dose 200 and 400mg/ kg body weight result was found to be gradually decreased in blood glucose level 75.75±1.92mg/dl and 72±2.73mg/dl at 180min from the study it was predicted that PHF possess Anti-hyperglycemic activity. Experimental study was shows that on repeated administration of PHF and glibenclamide for 14 days, a sustained and significant decrease in the average blood glucose level of diabetic rats was observed. End of the interventional period biochemical parameters were studied, and it was found to be level of SGOT and urea level remain constant at dose of 200mg/kg, decrease in SGPT is near to standard and decrease in creatinine level is greater than Std at dose of 400mg/kg. Conclusion: PHF containing extracts of (Azadirecta indica, Moringa oeifera and Andrographis paniculata) showed significant antidiabetic and antihyperlipidemic activity which was close to standard drug. Along with remarkable reduction in Total Cholesterol (TC) level and increased in High Density Lipoprotein (HDL) DXM induced diabetes rats. The formulation has emerged as potential combination which can challenge the synthetic drug.
Keywords: Diabetes mellitus; Azadirechta indica; Moringa oleifera; Andrographis paniculate; Polyherbal formulation, Glibenclamide
Journal Name :
EPRA International Journal of Research & Development (IJRD)

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Published on : 2022-08-03

Vol : 7
Issue : 8
Month : August
Year : 2022
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