In the article, the acclimatization of microclonal propagated cherry Krimsky-5 grafts in different nutrient environments was studied. In this case, grafts were grown on substrates such as "Agrobalt-S", "Agrobalt-V" peat and "Bioghumus" at an air temperature of 25oC and relative humidity of 65-70%. Also, growth and development of microplants in open field conditions, growth dynamics of height and body diameter, number of leaves, leaf surface and root system development indicators are highlighted.