Leptospira, a genus of pathogenic spirochetes, are the source of the infectious disease leptospirosis, which affects both humans and animals. It is connected to environments with inadequate sanitation and agricultural jobs that need contact with animals or water and is regarded as the most prevalent zoonosis in the world. Adventure travel and "mud run" sports or events that expose competitors to fresh water or soil are putting people at risk more and more. AIM:To assess the level of knowledge, attitude, and practice regarding leptospirosis prevention among the non-paramedical students. METHODS AND MATERIALS: A descriptive research design was conducted. 60 samples were selected by convenient sampling technique. Self - structured questionerwere used to collect data from the participants. RESULTS: The present study suggested that the26 (43.3%) had inadequate, 19 (31.7%) had moderate and 15 (25%) had adequate knowledge, 25(41.7%) had adequate, 16(26.7%)had moderate, 19(31.7%) had inadequate attitude, 22(36.7%) had inadequate, 13(21.7%) had moderate,25 (41.7%) had adequate practice. The occupationhad shown statistically significant association with level of knowledge regarding leptospirosis preventionat p<0.05.This study showed that the majority of respondents had low levels of knowledge, unfavorable attitudes, and poor practices regarding leptospirosis prevention. CONCLUSION:As a result, leptospirosis prevention knowledge, attitudes, and practices were generally substandard. They might be more likely to get leptospirosis as a result of this discovery