Background: The sanitary conditions and infrastructure of food establishments play a vital role in maintaining food safety and preventing contamination. Objective: This study evaluated the sanitation practices and infrastructural adequacy of food preparation environments in Khartoum Locality, Sudan. Methods: A cross-sectional observational study was conducted in (2020) among (34) food establishments, including restaurants, and cafeterias. Data were collected using structured checklists adapted from Codex Alimentarius guidelines, focusing on kitchen cleanliness, waste disposal, ventilation, water supply, and pest control. Results: Only (26.5%) of establishments met basic sanitation standards. Potable water was available in (97%] of sites, while proper waste disposal facilities were present in (55.9%). Ventilation was adequate in (64.7%), and signs of pest infestation were observed in (85.3%) of establishments. Conclusion: The study revealed substantial deficiencies in workplace sanitation and infrastructure in Khartoum Locality, which may contribute to food contamination and foodborne disease outbreaks.