This study investigated the effect of aqueous Tapinathus bangwensis on some diagnostic enzymes in alloxan induced diabetic wistar rats. The effect of plant extract were monitored on the serum concentrations as amylase (AMYL) aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), Lactate dehydrogenase (LDH) and creatinine kinas (CK) enzymes. Group 1 constitute the normal control which received only feed and water, group 2 received 50mg/kg citrate buffer. Alloxan was dissolved in 0.1M citrate buffer solution, PH 4.5. Group 1 constitute the normal control which received only feed and water, group 2 received 50mg/kg citrate buffer. Group 3 was administered alloxan solution and allowed free access to feed and water. Group 4-6 received 50mg/kg of citrate buffer and were also administered with the aqueous extract of Tapinanthus bangwensis at a dose of 250mg/kg and referred to as normal treated, concentration 1(NT conc-1), concentration 2 (NT con-2) and concentration 3 (NT conc- 3) respectively. Group 7-9 were administered 50mg/kg alloxan and different grades (5%, 7% and 10%) respectively of the aqueous extract. Blood, liver and pancreatic tissue samples were collected into appropriately labelled sample bottles and analysed. Blood, liver and pancreatic tissue samples were collected into appropriately labelled sample bottles and analysed. Result of the blood analysis showed that diagnostic enzymes were elevated significantly (p<0.05) in the group 3 rats. Oral administration of 250mg/kg aqueous Tapinathus bangwensis extract to group 7, 8 and 9 of 50mg/kg, 70mg/kg and 100mg/kg respectively significantly (p<0.05) decreased many of these biochemical alterations in a dose dependant manner. 100mg/kg administration of the extract showed the highest effect in lowering the elevated parameters followed by 70mg/kg administration. 50mg/kg had the least lowering effect. AMYL decreased in group 7, 8 and 9 respectively. Similarly, the respective decreases in group 7, 8 and 9 were for AST, ALT, LDH and CK. Histopathological results of the pancreas and liver also conformed to these chemical pathological results.