The purpose of this study was to find out the Isolated training group Combined impact of weight training group and ladder training group selected on muscular strength endurance and Breath Holding Time among inter collegiate female Kabaddi players. Sixty (60) female students from Dr.Sivanthi aditanar college of Engineering, Tiruchendur, Govindammal Aditanar College for women, Tiruchendur, Wavoo Wajeeha womens college of arts and science, veerapandipattanam were participant selected. The age of the participant was ranged from 18 to 25 years. The selected fitness variables Muscular strength endurance were assessed by using sit up test and the physiological variable Breath holding time were assessed by using nostril clip scoring seconds. The selected students were divided in to four groups namely Isolated training group Combined impact of weight training group and ladder training group and control group. Isolated training group Combined impact of weight training group and ladder training group underwent specific drills/exercise according to their training design which is instructed by the researcher whereas Control group underwent zero training. The obtained data were statistically analysed by ANCOVA was used to find out the significant difference among the group. An alpha level of 0.05 was used for all tests. The results indicate that there is significant difference between Control group, and three training groups Isolated training group Combined impact of weight training group and ladder training group Among the three training groups, Combined impact of weight training group shows better results compared to Isolated Training group and ladder training group.