The aim of the study was to develop a hand sanitizer that offers secure and efficient substitutes. Aloe Vera-based hand sanitizer that has no other potentially dangerous components. Aloe Vera is widely known for its ability to naturally hydrate skin, and its gel-like consistency makes it ideal for use in hand sanitizers. However, many people are unaware that Aloe Vera naturally protects against viral and bacterial illnesses because it contains polyphenols that stop bacterial growth. Aloe Barbadensis (Aloe Vera) Leaf Juice, which is made organically, serves to fight bacteria alongside alcohol without interfering with your hormonal balance. The purpose of the study is to 1.) To determine the properties and its compounds of Aloe Vera as potential Hand sanitizer, 2.) To determine the Quantity of different ingredients in preparation of Aloe Vera Hand Sanitizer, 3.) To test the Microbial Counts of Aloe Vera Hand Sanitizer with Different Essential Oils. The Study used organic Aloe Vera gel was tried as the major components of hand sanitizer, with an additional proportion of grain alcohol, distilled water and essential oils which give varied scent and effect to the hand sanitizer. Microbial test was done by the Department of Science and Technology (DOST) (Regional Standards and Testing Laboratory) to determine the number of microbes present in the hand sanitizer with different essential oils. In light of the findings, Aloe vera hand sanitizer with rose essential oil showed a less number of microbes, which was found similar with the commercial hand sanitizer.