Chimpanzees (Pan troglodytes) are well adapted great apes and best known for the pivotal role in hominid evolution. They are social animals like human. For the conservation purpose, these primates are kept into zoos throughout the world, and India is not an exception. The present study was carried out in The Zoological Garden, Alipore in Kolkata. They show various activities in the ex-situ conservation system. The present study was focused on the general diurnal activities of the adult chimpanzees in ex-situ condition and negative interactions of three adult chimpanzees to the visitors in the summer season (March- April, 2019). The observer found different activities with different anthropogenic cues as well as natural cues. Ad Libitum sampling technique was followed by the instantaneous scan sampling [1] for recording data. Each scan took five minutes with next five minute interval immediately. A mobile alarm (device: ASUS zenfone Max) was used for time maintenance to record the data perfectly. Total ten scans had been done per day to fulfill the objectives. Though the study is not concluding, but this type of effort is the very basic of observing the animals in ex-situ condition.