The objective of this study was to find out the effect of isotonic strength training on strength physiological and skill performance variables of men handball players. To achieve the purpose of this study 40 Intercollegiate handball players were selected from affiliated colleges of Bharathiar university, Coimbatore. The selected subjects were randomly divided into two groups such as Group I underwent isotonic strength (ISTG) (n=20) and Group II acted as control group (CG). The respective training was given to the experimental group for 3 days per week (Monday, Wednesday and Friday) for the period of sixteen weeks. The control group was not be given any sort of training except their routine work. The strength parameter leg strength were measures by wall squat test, physiological variables breath holding time were measures by breath holding fitness test and skill performance variables shooting ability was assessed by Zinn team handball skill battery test. The data collected from the subjects was statistically analyzed with t ratio to find out significant improvement if any at 0.05 level of confidence. The result of the leg strength, breath holding time and shooting ability improved significantly due to effect of isotonic strength training with the limitations of (diet, climate, life style) status and previous training the result of the present study coincide findings of the investigation done by different experts in the field of sports sciences.